Kombucha

During fermentation the symbiotic culture of bacteria and yeasts promotes the formation of various organic acids. Of these, gluconic acid plays a very important role because it is associated with the detoxification of heavy metals in the human body, having a significant impact on the organoleptic level. It is therefore essential to monitor it, not only during the fermentation process of the tea, but also, and above all, in the finished product in combination with acetic acid.

Determination of the Gluconic acid in Kombucha

CDR KombuchaLab the easy-to-use analysis system to optimise your kombucha production process by obtaining results in real time.

Test type: End Point. Time testing:[uni]. Analysis sessions with multiple samples are possible for up to a maximum of 16 tests for CDR KombuchaLab and 3 tests for CDR KombuchaLab Junior.<h3>Sample treatment</h3>For precise and repeatable analysis, degas the sample using the ultrasonic bath (code 222062) and clarify it, if necessary, using the centrifuge (code 222061).

Method

Gluconic acid is phosphorylated through the action of a suitable enzyme in the presence of ATP. The adduct formed through the action of NAD+ results in the formation of NADH in a 1:1 ratio with the gluconic acid present in the sample. The end point reaction is measured at 366 nm and the increase in absorbance is directly proportional to the concentration of gluconic acid.

Test Principle

The calibration curve of the CDR KombuchLab&nbsp;instrument, obtained by comparing the CDR method with the enzymatic method for the determination of gluconic acid, confirmed an excellent linearity of the system over the entire measurement range.

Calibration Curve

Calibration curve of the CDR KombuchaLab instrument, obtained by comparing the CDR method with the enzymatic method for the determination of gluconic acid. R² = 0.9804

Reagent test Kits

Measuring range

CDR KombuchaLab: the easy-to-use analysis system to optimise your kombucha production process by obtaining results in real time. Top version

CDR KombuchaLab

CDR KombuchaLab: the easy-to-use analysis system to optimise your kombucha production process by obtaining results in real time. Junior version